316-TIRTL-seq: 深度定量 TCR 链配对新法

该文介绍了一种名为TIRTL-seq的创新实验和计算方法，旨在实现深度、定量且经济高效的T细胞受体（TCR）配对测序。传统的TCR测序方法往往需要在低成本的批量测序（缺乏配对信息）和昂贵的单细胞技术（提供配对信息但通量有限）之间做出取舍。TIRTL-seq通过使用384孔板形式进行组合分析，大大降低了成本，使其适用于大规模队列研究。在计算方面，该方法不仅采用了MAD-HYPE算法，还开发了T-SHELL启发式算法，成功解决了对高丰度克隆型进行链配对的难题。与10x Genomics等现有技术相比，TIRTL-seq在成本相似的情况下，能够识别出多达百倍的配对克隆型。该技术被成功应用于追踪一名患者在SARS-CoV-2和EBV感染期间表现出的不同T细胞克隆动态，证明了其在纵向免疫图谱分析中的强大功能。References:* Pogorelyy M V, Kirk A M, Adhika...去小宇宙查看完整单集简介前往小宇宙评论区与主播互动

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