Let's Talk Micro

Recorded live at ASM Microbe 2026 in Washington, DC, this special episode marks the first live recording of Let's Talk Micro. Joining me are co-host Dr. Andrea Prinzi and guest Dr. Nathan Ledeboer for a discussion on the evolving role of whole genome sequencing (WGS) in clinical microbiology and antimicrobial resistance. Can whole genome sequencing predict antimicrobial susceptibility? What are its current limitations? How close are we to incorporating WGS into routine clinical microbiology laboratories? Throughout the conversation, we explore the opportunities and challenges of implementing sequencing technologies, including bioinformatics, workflow, reimbursement, and the need for continued collaboration between microbiologists, infectious diseases specialists, pharmacists, and antimicrobial stewardship teams. We also discuss how WGS may transform outbreak investigations, epidemiologic surveillance, and the future role of the clinical microbiologist as advanced diagnostics become increasingly integrated into patient care. Whether you're a bench microbiologist, laboratory leader, infectious diseases professional, or student, this episode offers an exciting look at where clinical microbiology is headed and what whole genome sequencing can realistically tell us about antimicrobial resistance. Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

En este episodio en español de Let's Talk Micro, Luis conversa nuevamente con el Dr. Germán Esparza sobre los cambios más relevantes incluidos en la edición 2026 del documento CLSI M100. Durante la conversación, revisan nuevas opciones terapéuticas como aztreonam-avibactam, actualizaciones en los puntos de corte para Acinetobacter y Neisseria gonorrhoeae, cambios en aminoglucósidos, consideraciones de control de calidad y los desafíos asociados con las pruebas de susceptibilidad para el complejo Burkholderia cepacia. Además, discuten cómo los laboratorios pueden evaluar e implementar estos cambios de manera segura, la importancia de la colaboración con los programas de optimización de antimicrobianos y ofrecen un adelanto de la próxima edición del documento CLSI M45. Temas discutidos: Aztreonam-avibactam y resistencia antimicrobiana Actualizaciones de puntos de corte y pruebas de susceptibilidad Control de calidad e implementación de cambios en el laboratorio Avances y perspectivas para CLSI M45 🔗 Recursos Acceda gratuitamente al CLSI M100: https://em100.edaptivedocs.net/Login.aspx Manténgase conectado con Let's Talk Micro: Página web: letstalkmicro.com ¿Preguntas o comentarios? Escríba a [email protected] ¿Le interesa participar como invitado en Let's Talk Micro? Complete el siguiente formulario: https://forms.gle/V2fT3asjfyusmqyi8 Apoya el pódcast: Venmo Buy me a Ko-fi  

ASM Microbe is the largest microbiology meeting in the United States, bringing together thousands of scientists, laboratorians, clinicians, and industry partners to discuss the latest advances in microbiology. In this episode, Luis shares highlights from ASM Microbe 2026, including sessions on diagnostic challenges, antimicrobial resistance, rapid susceptibility testing, emerging therapeutics, laboratory automation, phage therapy, and whole-genome sequencing. Topics include a Brucella case that reinforces the importance of Gram stain interpretation, updates on resistance mechanisms in Pseudomonas aeruginosa and KPC-producing organisms, advances in rapid AST, and innovations showcased in the vendor hall. Luis also discusses several posters that caught his attention, covering pharyngeal gonorrhea, direct-from-specimen susceptibility testing, phage testing, rapid resistance reporting for Mycobacterium abscessus, and CRISPR-based diagnostics for bloodstream infections. Whether you attended ASM Microbe 2026 or couldn't make it this year, this episode provides a practical overview of some of the trends, technologies, and conversations shaping the future of clinical microbiology. Topics Discussed: • Diagnostic challenges in clinical microbiology • Rapid identification and susceptibility testing • Antimicrobial resistance and emerging therapies • ESKAPE pathogens and Pseudomonas aeruginosa • Laboratory automation and AI-assisted workflows • Phage therapy and phage susceptibility testing • Whole-genome sequencing and CRISPR diagnostics Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

What does it take to go from working at the bench to becoming a future clinical microbiology laboratory director? In this episode, Luis is joined by Dr. Mackenzie Collins, a CPEP fellow at UCLA, to discuss her journey from Medical Laboratory Scientist (MLS) to PhD scientist and CPEP fellow. Mackenzie shares how she discovered laboratory medicine, her experience working as an MLS in molecular microbiology, and the mentors and opportunities that helped shape her career path. The conversation explores the CPEP fellowship, the role of laboratory directors, and how MLS professionals can strengthen their applications through projects, research, presentations, and professional involvement. Mackenzie also discusses her PhD work involving HIV reservoirs and long-read sequencing, and why genomics and molecular diagnostics will continue to play an increasingly important role in clinical microbiology. Whether you're considering graduate school, interested in the CPEP fellowship, or exploring career paths in laboratory medicine, this episode offers a firsthand look at one MLS's journey from the bench to clinical microbiology leadership. Topics discussed: The MLS-to-CPEP pathway and transitioning from the bench to graduate school Mentorship, professional development, and building a competitive application The role of clinical microbiology laboratory directors and career opportunities beyond the bench Sequencing, genomics, and the future of clinical microbiology Additional Resources CPEP Fellowship Overview with Dr. Sam Goodfellow Interested in learning more about the CPEP Fellowship? In this episode, Dr. Sam Goodfellow discusses the structure of the program, the application process, and what to expect from CPEP training. https://directory.libsyn.com/episode/index/id/36395120 Understanding the ABMM Certification Examination Learn more about the American Board of Medical Microbiology (ABMM) certification, its importance for future laboratory directors, and what candidates can expect from the examination process. https://directory.libsyn.com/episode/index/id/35868345 Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

Have you ever received an identification result that just didn't make sense? In this episode, Luis shares three cases of mistaken identity from the microbiology laboratory: a Gram-positive rod identified as Kocuria, another reported as Sphingomonas, and a yeast that somehow became Brucella. While automated identification systems are powerful tools, they can only work with the information we provide. These examples highlight an important lesson for every microbiologist: always correlate the identification with the Gram stain, colony morphology, and other bench observations. When the result doesn't fit, take a step back and ask a simple question: Does it make sense? Join us for a quick Micro Minutes on critical thinking, correlation, and why the Gram stain remains one of the most valuable tools in the microbiology laboratory. Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

In Part 2 of this hantavirus series, Luis continues the conversation with Dr. Ryan Relich and Dr. Stephanie Seifert by focusing on the recent Andes virus cruise ship outbreak and what makes this hantavirus different from others. The discussion explores the outbreak investigation, the evidence for person-to-person transmission, and why the Andes virus has received so much attention from the public health community. Ryan and Stephanie also discuss diagnostic testing, including PCR and serology, the role of public health laboratories, current treatment options, prevention strategies, and how concerned the public should be about future outbreaks. Tune in for a closer look at Andes virus and its role in the recent cruise ship outbreak. Stay updated: https://www.cdc.gov/hantavirus/situation-summary/index.html Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

Hantaviruses have recently been in the headlines, but what exactly are they and how do they spread? In Part 1 of this hantavirus series, Luis is joined by Dr. Ryan Relich and Dr. Stephanie Seifert for a deep dive into the world of hantaviruses. The conversation explores the basics of hantaviruses, including their ecology, rodent reservoirs, transmission, and the diseases they cause in humans. The guests also discuss Old World vs New World hantaviruses, hantavirus pulmonary syndrome (HPS), environmental risk factors, climate influences, and why these infections may be underdiagnosed. From deer mice and spillover events to aerosol transmission and public health concerns, this episode breaks down the science behind these important zoonotic viruses in a practical and approachable way. Stay tuned next week for an episode where our guests talk about the Andes virus.   Stay updated: https://www.cdc.gov/hantavirus/situation-summary/index.html Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

Workforce shortages continue to impact clinical laboratories across the country, especially in rural healthcare settings. In this episode of Let's Talk Micro, Luis is joined by Kenzi Haigler to discuss an innovative high school laboratory program designed to introduce students to laboratory medicine early and help strengthen the future workforce pipeline. Kenzi shares her journey into the laboratory profession, her experiences working in rural healthcare, and the unique challenges small hospitals face when it comes to staffing, training, and retaining laboratory professionals. She also explains how her community came together to create a hands-on clinical laboratory assistant program that gives students exposure to phlebotomy, microscopy, waived testing, and laboratory workflows before graduation. This conversation highlights the importance of mentorship, early exposure to laboratory medicine, and creative approaches to workforce development in clinical laboratory science. Follow Kenzi on TikTok:  @lablifewithmrsh Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

In this episode of Let's Talk Micro, we close out our gram-negative cocci series by talking about the "background singers" of the group—the organisms that quietly show up on respiratory cultures and oral flora plates every day. We explore the world of non-pathogenic Neisseria species, along with rod-shaped and coccobacillary Neisseria and Moraxella that break the classic gram-negative diplococci rule. From commensals and opportunistic pathogens to their role in respiratory cultures and bench identification, this episode focuses on how to think through these isolates in the clinical microbiology laboratory. As always, we tie it back to the bench—because sometimes the organisms sitting quietly in the background still matter.   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

This week, Let's Talk Micro is pausing the Gram-negative cocci series to talk about one of the biggest microbiology meetings of the year: ASM Microbe 2026. Luis is joined by Dr. Bobbi Pritt and Dr. Robert Tibbetts to discuss what attendees can expect from this year's meeting in Washington, DC. The conversation covers the new meeting structure, important trends in clinical microbiology, sessions and workshops geared toward medical laboratory scientists, networking opportunities, and tips for getting the most out of the conference experience. They also discuss hot topics such as AI-driven diagnostics, whole genome sequencing, metagenomics, phage therapy, antimicrobial resistance, and the future direction of clinical microbiology. Whether you are attending ASM Microbe for the first time or returning as a seasoned attendee, this episode offers a practical preview of what's ahead and highlights opportunities especially relevant to the clinical microbiology laboratory community. Topics discussed: Changes to ASM Microbe 2026 Sessions and workshops for MLS professionals AI and digital diagnostics Whole genome sequencing and metagenomics Antimicrobial resistance and AST Phage therapy and microbiome-focused sessions Networking and professional development Tips for first-time attendees ASM Resources ASM Microbe 2026 information and registration: https://registration.experientevent.com/ShowASM261 Volunteer opportunities with ASM: https://asm.org/about-asm/volunteer-with-asm/volunteer-with-asm MLS/MLT workshop information and registration: https://www.pathlms.com/asm/courses/185029 Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

In this episode of Let's Talk Micro, we continue our gram-negative cocci series with a closer look at Moraxella catarrhalis—better known at the bench as the hockey puck bug. We break down what makes this organism unique, from its classic sliding colony morphology to how it behaves in different patient populations. Is it a colonizer or a pathogen? The answer depends on the clinical context. We walk through its role in pediatric infections like otitis media and sinusitis, its significance in adults—especially in COPD—and how to interpret its presence in respiratory cultures. As always, we tie it back to the bench, discussing identification clues, culture considerations, and what it means when you see this organism on your plate. Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

In this week's episode, we continue our series on gram-negative cocci and take a closer look at two of the most important organisms: Neisseria meningitidis and Neisseria gonorrhoeae. On the Gram stain, they may look the same—but clinically, they represent very different scenarios. We walk through colonization, transmission, and key virulence factors, and connect them to the infections they cause—from meningitis and invasive disease to mucosal and disseminated gonococcal infections. We also bring it back to the bench, discussing specimen source, testing approaches like NAAT and culture, and how to think through these organisms in real-world laboratory settings.   Looking to stay up to date with the latest clinical microbiology resources? Check out Wiley's ClinMicroNow—bringing trusted ASM Press content into one powerful, searchable platform : 🔗 https://onlinelibrary.wiley.com/doi/book/10.1002/9781683670438?utm_source=extweb&utm_medium=referral&utm_campaign=lets_talk_micro_podcast_ptnr&utm_content=mid-roll-ad&utm_term=clinmicronow   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

In this episode we continue our gram-negative cocci series by moving beyond the plate and into modern identification methods. From traditional systems like VITEK and RapID NH to MALDI-TOF and molecular testing, we explore how these organisms are identified in today's clinical microbiology lab. We also break down the role of NAATs and syndromic panels in detecting organisms like Neisseria meningitidis and Neisseria gonorrhoeae, especially in situations where culture may be delayed or negative. This episode focuses on how these tools fit into real-world workflows—highlighting their advantages, limitations, and how to interpret results at the bench. Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

In this episode of Let's Talk Micro, we continue our gram-negative cocci series by focusing on what to do after the Gram stain. You've identified gram-negative cocci—now what? We take a practical, bench-focused approach to working these organisms up, covering key culture media like blood agar, chocolate agar, and selective media such as Modified Thayer-Martin and Martin-Lewis. We also review collection and transport considerations, including JEMBEC and CO₂ requirements for Neisseria gonorrhoeae. We then dive into commonly used tests, including ONPG for differentiating Neisseria lactamica and Neisseria meningitidis, the CTA test and its limitations, and the butyrate esterase test for identifying Moraxella catarrhalis. This episode is all about building confidence at the bench—connecting what you see to what you do next.   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

In this episode of Let's Talk Micro, we kick off a new series on gram-negative cocci by starting where it all begins—the Gram stain. Seeing gram-negative diplococci on a smear can feel intimidating, especially when you're on the bench and need to make the right call. In this episode, we focus on how to approach that moment with confidence. We walk through the key organisms you'll encounter, including Neisseria, Moraxella, and Veillonella, and discuss how morphology, specimen source, and clinical context all come together to guide your thinking. We also revisit a correction from a previous episode regarding carbohydrate utilization in Neisseria species. This episode is all about building a strong foundation—how to think before jumping to identification. Stay tuned for the next episode, where we'll dive into the media and tests used to identify these organisms in the lab. Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

The CLSI M100 gets updated every year—but what do those changes actually mean for the microbiology lab? In this episode of Let's Talk Micro, Luis is joined by Dr. April Bobenchik to break down the latest updates in the CLSI M100 2026 (36th edition) and how they translate into real-world lab practice. They walk through important breakpoint changes, including aztreonam–avibactam and updates for Acinetobacter, along with new additions like trimethoprim-sulfamethoxazole breakpoints for beta-hemolytic streptococci. Another notable update this year is the updated anaerobic antibiogram and how it can be used to help guide decisions in the lab. The conversation also touches on antimicrobial tiers, why some breakpoints are temporarily removed, and practical ways labs can approach implementing new updates. If you work in clinical microbiology, this episode will help you stay current and confident with the latest M100 changes.   🔗 Resources Access CLSI M100 for free: https://em100.edaptivedocs.net/Login.aspx Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

Luis here with a quick update! We're taking a short break and will return the week of March 23rd. During this time, it's a great opportunity to explore the archive—Let's Talk Micro has over 200 episodes covering many topics in clinical microbiology. New episodes will be back soon. Thanks for listening and for being part of the Let's Talk Micro community. Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

Wound cultures are some of the most complex specimens encountered in the clinical microbiology laboratory. Unlike more straightforward cultures, wound specimens often contain multiple organisms, making it challenging to determine which microbes are clinically significant and which represent colonization. In this episode of Let's Talk Micro, Luis is joined by Dr. Christopher Doern and Dr. Alexandra Bryson to discuss the wound culture guidelines from the Clinical Microbiology Procedures Handbook (CMPH) and how they can help laboratorians navigate these challenging cultures. Together, they explore practical approaches to interpreting polymicrobial wound cultures, deciding which organisms to work up, and balancing thorough reporting with antimicrobial stewardship. The discussion also covers specimen collection challenges, the role of Gram stains in evaluating specimen quality, guidance on working up common wound pathogens, and strategies to streamline laboratory workflows. Whether you're a student, a new microbiology technologist, or an experienced laboratorian, this episode offers practical insights to help you confidently approach wound cultures and apply CMPH guidance at the bench.   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

The Gram stain is one of the most trusted tools in clinical microbiology—but it doesn't always tell the full story. In this Micro Minutes episode, we break down why Gram stains can sometimes be misleading and how factors like culture age, antibiotic exposure, decolorization, morphology, and arrangement can change what you see under the microscope. We also touch on classic troublemakers like Acinetobacter and tiny coccobacilli such as Pasteurella and Haemophilus, and how careful observation can keep you from jumping to the wrong conclusion. Short, focused, and straight from the bench—this episode is all about learning how to interpret Gram stains beyond just purple and pink.   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

In this episode of Let's Talk Micro, we step beyond the microbiology bench to talk laboratory medicine, advocacy, and storytelling with fellow medical laboratory scientists and podcasters Stephanie Whitehead and Aaron Odegard. We explore how their work on the SuperStat Podcast is helping amplify the voice of the laboratory through conversations on leadership, access, and visibility. The discussion also highlights Little Lab Learners: ABCs of Laboratory Medicine, a children's book created to introduce laboratory medicine early and inspire future lab professionals. Together, we talk about lab career paths, the impact of automation and the pandemic on lab visibility, and why advocacy doesn't look the same for everyone—but matters now more than ever. This episode is a reminder that the lab's story doesn't end at the bench—it's one we all help tell.   📘 Learn More About Little Lab Learners Little Lab Learners: ABCs of Laboratory Medicine is a children's book created to introduce laboratory medicine early and help kids see lab professionals as scientists, problem-solvers, and essential members of healthcare. Learn more about the book and the Little Lab Learners series here: 👉 https://labcares.org/Little_Lab_Learners/   🎙️ Check Out the SuperStat Podcast Listen to the SuperStat Podcast on Spotify: 👉 https://open.spotify.com/show/5wYrCFh375KclnCFY8YyN2?si=b681fc3748df48e4   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8   Support the podcast: Venmo Buy me a Ko-fi  

In this episode of Let's Talk Micro, Luis is joined by Dr. Joel Mortensen to discuss a study examining intestinal parasites in pediatric patients in Guinea, West Africa. They dive into the real-world challenges of diagnosing parasitic infections in resource-limited settings, including reliance on stool microscopy, the use of rapid antigen tests, and how seasonal changes influence infection rates. Dr. Mortensen walks us through the study design, key findings, and what the data revealed about protozoal infections, as well as the role of microscopy in identifying helminths in this setting. This conversation highlights the critical role of clinical microbiology in global health, the importance of diagnostic context, and how laboratory data can directly inform patient care and public health strategies in underserved regions.   Check out Dr. Joel Mortensen's previous episodes If you enjoyed this conversation, be sure to check out previous episodes featuring Dr. Mortensen, where we dive deeper into his work in Guinea, laboratory capacity building, and global health challenges in resource-limited settings: 🎙️ Episode 107: A Pediatric Hospital in Guinea – Pt. 1 https://directory.libsyn.com/episode/index/id/35868465 🎙️ Episode 108: A Pediatric Hospital in Guinea – Pt. 2 https://directory.libsyn.com/episode/index/id/35868460 These episodes provide important background on the hospital, training efforts, and the broader impact of this work. 🔗 Other Links Learn more about Sacre Coeur Hospital and the pediatric care and laboratory work being done in Guinea: 👉 https://www.hopeignited.org/sacre-coeur 📩 Want to get involved or learn more? If you're interested in learning more about this work, collaborating, or supporting efforts in Guinea, you can reach out directly to Dr. Joel Mortensen: 📧 [email protected] You can also contact me at [email protected], and I'm happy to help connect you. Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

Genomic epidemiology is reshaping how we detect, investigate, and control infectious disease outbreaks—but what does that actually look like from the bench to public health? In this episode of Let's Talk Micro, Luis is joined by genomic epidemiologist Krisandra Allen for a practical, case-based conversation on how pathogen sequencing data is generated, analyzed, and paired with epidemiologic information to answer questions traditional methods can't. They discuss foodborne and hepatitis A outbreaks, geographically dispersed clusters, and how sequencing is being integrated into routine public health workflows, while highlighting the essential role of clinical microbiology labs in specimen submission and data quality. A timely episode for microbiologists, public health professionals, and trainees curious about how their lab work feeds into real-world surveillance and outbreak response.   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8   Support the podcast: Venmo Buy me a Ko-fi  

In this episode of Let's Talk Micro, Luis is joined by faculty and collaborators from the University of Florida to discuss their new Clinical Laboratory Microbiologist (CLM) program — the first NAACLS-approved, microbiology-only certification pathway in the United States. They share how the program was created to address the growing shortage of clinical microbiologists and provide a direct pathway for microbiology graduates and working professionals to enter the clinical laboratory. The conversation covers: Gaps in the current workforce and training pipeline The hybrid model combining online coursework, hands-on bootcamp labs, and local clinical internships Real-world training with clinical lab technology such as MALDI-TOF, PCR, and blood culture systems  Preparation for the ASCP categorical microbiology certification exam The strong nationwide interest in the program The episode closes with a fun discussion on everyone's favorite microbes. Whether you're a student, lab professional, or educator, this episode offers insight into the future of clinical microbiology training.   Additional resources: ASCP Categorical Certification (Microbiology) https://www.ascp.org/boc/explore-credentials/view-all-credentials/M University of Florida Clinical Laboratory Microbiologist (CLM) Program https://microbiologyonline.ifas.ufl.edu/programs/clinical-laboratory-microbiologist/ GIDEON (Global Infectious Diseases and Epidemiology Network) https://www.gideononline.com/ Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

Tick-borne diseases continue to expand across North America, but diagnosing them in the clinical laboratory remains complex—especially when timing between symptom onset and testing isn't considered. In this episode of Let's Talk Micro, Luis is joined by Kyle Rodino, Assistant Professor of Pathology and Laboratory Medicine at the University of Pennsylvania and Assistant Director of Microbiology, for a lab-focused discussion on tick-borne disease diagnostics. They cover major bacterial, parasitic, and viral causes of tick-borne disease, and walk through how molecular testing, serology, and microscopy are used at different stages of illness. The conversation highlights why test selection and timing matter, common diagnostic challenges—particularly with Babesia and Lyme disease testing—and how laboratories can support better diagnostic decision-making. A practical episode for microbiologists, laboratorians, and clinicians navigating real-world testing challenges in the lab.   Additional resources: Update on North American tick-borne diseases and how to diagnose them https://doi.org/10.1128/jcm.00807-23   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8   Support the podcast: Venmo Buy me a Ko-fi  

In this episode of Let's Talk Micro, we break down metagenomic next-generation sequencing (mNGS) and how it's changing the way we diagnose complex infectious diseases. I'm joined by Steve Miller, MD, PhD—Chief Medical Officer at Delve Bio—to discuss how unbiased metagenomics moved from research labs into real-world clinical practice. We cover what metagenomics is, how it differs from targeted PCR and sequencing, and where it adds the most value—especially in meningitis and encephalitis, immunocompromised patients, and cases where routine testing comes back negative. Dr. Miller shares insights from years of clinical experience, including how mNGS can improve diagnostic yield, shorten time to diagnosis, guide targeted therapy, and reduce unnecessary testing and hospital stays. We also touch on challenges like cost, result interpretation, diagnostic stewardship, and where metagenomics is headed next—including its role in public health and emerging infections.    Links & Resources Clinical metagenomics for meningitis and encephalitis (Nature Medicine) https://www.nature.com/articles/s41591-024-03275-1    Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

PEA agar is often treated as "the gram-positive plate," but that shortcut can lead to real bench-level mistakes. In this MicroMinutes episode, we break down what phenylethyl alcohol agar actually does, why growth on PEA does not automatically mean an organism is gram-positive, and how selective media can change colony appearance and behavior. You'll hear about: What PEA inhibits — and what it doesn't Why Pseudomonas aeruginosa can grow quite well on PEA How colony morphology and hemolysis may differ from blood agar Why Lancefield grouping should never be performed directly from PEA PEA is a powerful recovery tool — but only when you understand its limitations. Short, focused, and straight from the bench, this is your Micro minutes.   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8   Support the podcast: Venmo Buy me a Ko-fi  

In this special episode, I'm joined by three leaders who helped shape the conversation at the 2025 Antimicrobial Resistance (AMR) Summit in Detroit: Dr. Lauren Hunt, Dr. Linoj Samuel, and Dr. Robert Tibbetts. Together, we explore how this intimate, multidisciplinary summit brought together clinical microbiology, infectious diseases, stewardship, public health, policy, and veterinary medicine to tackle the growing challenges of antimicrobial resistance. We break down the key themes that emerged throughout the meeting—shared accountability, One Health collaboration, diagnostic innovation, payer perspectives, and the urgent need for equitable access to testing. You'll hear about surprising insights, personal stories that brought AMR's impact into sharp focus, and the collaborative projects already taking root as a result of this year's summit. Whether you're on the bench, managing stewardship programs, shaping policy, or simply passionate about the future of diagnostics, this episode offers a behind-the-scenes look at the conversations and partnerships driving AMR action forward. Thank you to Lauren, Linoj, and Robert for joining me—and to everyone committed to advancing the fight against AMR. One Health, one fight.    Additional Resources from the 2024 AMR Summit If you'd like to explore more background from last year's summit, here are two excellent resources: 2024 AMR Summit Article Antimicrobial Stewardship & Healthcare Epidemiology (ASHE) DOI:10.1017/ash.2025.10039 ASHE Podcast Episode Recapping the 2024 Summit 🎧 https://open.spotify.com/episode/0YBcDwgfYIy75pTVjCZZ5Y?si=jb8N_3AIQdOmcC2YaOvOag    Want to Contribute or Get Involved? If you're interested in contributing ideas, collaborating on AMR initiatives, or learning more about future AMR Summits, you can reach out directly to: Dr. Lauren Hunt  📧 [email protected]   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi   Support the podcast: Venmo Buy me a Ko-fi  

What does antimicrobial resistance look like when your patients range from dogs and cats to cattle, wildlife, and everything in between? And how do we make sense of AST, breakpoints, contamination, and genotypic tools across such a diverse microbiological world? In this episode, Luis is joined by Dr. Kelli Maddock, DrPH (North Dakota State University Veterinary Diagnostic Laboratory) and Dr. Stephen Cole (University of Pennsylvania), two leaders shaping the future of veterinary microbiology, antimicrobial stewardship, and CLSI veterinary standards. Together, they explore AMR and AST through a true One Health lens, showing how animal, human, and environmental health are interconnected in everyday diagnostic work. Drawing from their recent American Journal of Veterinary Research article on AMR challenges in veterinary medicine, the discussion highlights: The biggest challenges in veterinary AST, from species diversity to contamination Phenotypic vs genotypic testing and concerns around WGS-based "treatment predictions" How selective reporting ("nudging") strengthens antimicrobial stewardship Why every animal result matters in a One Health framework Whether you come from human or veterinary microbiology, this episode reveals the shared challenges across sectors — and the diagnostic, collaborative, and educational steps needed to strengthen AMR response in a One Health world. Link to article: https://doi.org/10.2460/javma.23.12.0687 Missed the past episodes on veterinary microbiology? Here they are: TBT: Veterinary Microbiology https://directory.libsyn.com/episode/index/id/35868175 Episode 166: Pasteurella multocida — capsular groups and more https://directory.libsyn.com/episode/index/id/35868155 Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

In this final episode of our special four-part series in partnership with bioMérieux, we bring all the pieces together. Throughout the series, we explored the diagnostic pathway from multiple angles: Episode 1: The evolution of antimicrobial susceptibility testing (AST) Episode 2: Molecular diagnostics for bloodstream infections Episode 3: FAST AST and the impact of getting susceptibility data hours sooner Today, in Episode 4, we shift to diagnostic stewardship and lab optimization — the strategies that ensure the right tests are used at the right time, and that workflows support efficient, meaningful, and actionable results. Luis is joined by Alyssa Walker, Infectious Diseases Pharmacist and Medical Advisor, and Jenny Harder, Lab Optimization Consultant with experience in managing a microbiology laboratory. Together, they explore how smarter workflows and better diagnostic decisions amplify the value of every ID and AST result. In This Episode: What diagnostic stewardship really means — right test, right patient, right time How lab optimization streamlines workflow, reduces waste, and improves turnaround times Key challenges labs face today (staffing, training, legacy processes) and how to address them How tools like BioFire Fireworks and Syndromic Trends enhance decision-making and support stewardship By connecting stewardship principles with optimized workflows, labs can turn rapid diagnostics into real clinical impact — faster decisions, smarter therapy, and better patient outcomes. This episode is sponsored by bioMérieux, a global innovator in infectious disease diagnostics. Missed the earlier episodes in this bioMérieux series? Here they are: Episode 198 – Let's Talk: The Evolution of AST Episode 204 – In the Bloodstream: Diagnosing Infections Faster Episode 211 – At the Speed of AST: Diagnosing Resistance Faster This episode is sponsored by bioMérieux, a global innovator in infectious disease diagnostics. Resources from this episode: bioMérieux Sepsis Webinar https://info.biomerieux.com/clin_us_2024_ams_handbook?utm_source=podcast&utm_medium=show-notes&utm_campaign=LetsTalkMicro Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

In this Micro Minutes episode, Luis breaks down classic microbiology traits that usually hold true, but not always.  From indole-negative E. coli to non-swarming Proteus and oxidase-negative Pseudomonas, this quick episode highlights real-world exceptions that can catch techs and students off guard. Learn how to spot: Indole-negative E. coli (98% rule + inactive biotypes) Lactose-fermenting look-alikes like Citrobacter freundii Proteus species that don't swarm Pseudomonas species that test oxidase negative A fast, practical reminder that no single biochemical test should stand alone.   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8 Support the podcast: Venmo Buy me a Ko-fi  

Welcome to the very first Micro Minutes, a new quick-hit series from Let's Talk Micro designed for students, techs, and anyone who wants fast, practical microbiology insights in under 10 minutes. In this episode, we tackle a common misconception that microbiologists can make at the bench when interpreting a single plate: MacConkey agar. Many assume: "If it doesn't grow on MacConkey, it's not a gram-negative rod." "If it does grow on MacConkey, it must be a gram-negative rod." Both are wrong — and today we break down why.   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8   Support the podcast: Venmo Buy me a Ko-fi  

In the third installment of our special series in partnership with bioMérieux,  Luis welcomes back Dr. Sonali Kalathiya, infectious diseases pharmacist and medical advisor at bioMérieux, to explore how innovation is accelerating antimicrobial susceptibility testing (AST). Together they dive into FAST AST—a phenotypic susceptibility test that delivers actionable results directly from positive blood cultures in hours instead of days. They discuss how this technology works, how it complements molecular testing, and how it's transforming patient care, antimicrobial stewardship, and laboratory workflows. From implementation and training to real-world clinical impact, this episode uncovers what it truly means to diagnose resistance at the speed of AST. 🎧 Tune in to learn how faster results are helping clinicians act sooner, optimize therapy, and improve patient outcomes. This episode is sponsored by bioMérieux, a global innovator in infectious disease diagnostics.   Resources from this episode: bioMérieux Sepsis Webinar https://info.biomerieux.com/clin_us_2024_ams_handbook?utm_source=podcast&utm_medium=show-notes&utm_campaign=LetsTalkMicro   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Interested in being a guest on Let's Talk Micro? Fill out the form here: https://forms.gle/V2fT3asjfyusmqyi8   Support the podcast: Venmo Buy me a Ko-fi  

Just back from IDWeek 2025 in Atlanta, Luis shares the sessions and posters that defined this year's meeting — from next-generation sequencing and diagnostic stewardship to AI in the microbiology lab and new antimicrobials on the horizon. Tune in for insights on: NGS test utilization and stewardship New β-lactamase inhibitors like nacubactam  AI's growing role in clinical microbiology Tedizolid and the importance of optimizing existing agents 🎧 Whether you were in Atlanta or following from afar, this episode captures the energy and innovation of IDWeek — and what it means for the future of diagnostics, stewardship, and patient care. Resources Learn more about ID Week : https://idweek.org/ Infectious Diseases Society of America: https://www.idsociety.org/   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Support the podcast: Venmo Buy me a Ko-fi  

En este episodio en español de Let 's Talk Micro, el Dr. Germán Esparza Sánchez , microbiólogo clínico, miembro del CLSI y consultor de la OPS/OMS, comparte las actualizaciones más recientes del documento M100 y cómo impactan la práctica microbiológica. Desde la eliminación de puntos de corte para Burkholderia cepacia hasta los nuevos antibióticos como aztreonam-avibactam, durlobactam-sulbactam y cefiderocol, analizamos los retos de aplicar las guías del CLSI en los laboratorios latinoamericanos, las diferencias epidemiológicas regionales y la importancia de la educación continua para combatir la resistencia antimicrobiana (RAM). Además, el Dr. Esparza comenta sobre el trabajo regional que realizan organizaciones como la Asociación Panamericana de Infectología (API) y COLABIOCLI, que promueven la colaboración científica y la mejora de los laboratorios en América Latina.  Enlaces mencionados Asociación Panamericana de Infectología (API) COLABIOCLI Organización Panamericana de la Salud (OPS) Organización Mundial de la Salud (OMS)   Conéctate con Let's Talk Micro: Página web: letstalkmicro.com ¿Preguntas o comentarios? 📩 Escríbeme a [email protected] Apoya el pódcast: Venmo Buy me a Ko-fi  

Puerto Rico is entering a new era in microbiome research. In this episode,  Luis speaks with Dr. Filipa Godoy-Vitorino about the newly established Microbiome Center of Puerto Rico — a hub designed to strengthen research, collaboration, and training across the Caribbean. Dr. Godoy-Vitorino shares how the center is helping scientists access expertise in bioinformatics and data analysis, offering bilingual workshops, and expanding representation of Caribbean populations in global microbiome studies. She also discusses the center's vision to connect with international partners, support local innovation, and create opportunities for the next generation of researchers. 🎧 Tune in to learn how this center is putting Puerto Rico on the global microbiome map.    Resources and articles mentioned: The Microbiota Vault: microbiotavault.org ISME (International Society for Microbial Ecology): isme-microbes.org ISALA Project (University of Antwerp): isala.be "Solutions to expand microbiome sciences in the Caribbean Region: an insider's perspective" by Filipa Godoy-Vitorino — Trends in Microbiology, 2025. View PDF / Article: ScienceDirect link ScienceDirect   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Support the podcast: Venmo Buy me a Ko-fi  

In this second half of our discussion with Dr. Erin McCreary and Dr. Hannah Creager, we dive deeper into how microbiology and antimicrobial stewardship teams collaborate to improve patient outcomes. They unpack how to prioritize breakpoint updates, the pros and cons of cascade reporting, and what nudges can do to guide better prescribing. Then we explore a powerful example of collaboration—the transition from MRSA agar to PCR across hospitals—and what it taught both teams about communication, resource sharing, and patient safety. Finally, Erin and Hannah reflect on lessons learned, first steps for building collaboration, and even share their favorite microbes (spoiler: both picked viruses!). 🎧 Tune in to learn how intentional teamwork between the lab and stewardship can transform patient care and lab efficiency.   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Support the podcast: Venmo Buy me a Ko-fi  

Good communication isn't optional—it's essential for patient care. In Part 1 of this two-part series, Dr. Erin McCreary and Dr. Hannah Creager join Luis to talk about how microbiology labs and antimicrobial stewardship teams can collaborate more effectively. They share real-world examples from their system-wide work—aligning AST panels and breakpoints across multiple platforms, navigating reporting challenges like the clindamycin D-test and rifampin comments, and building consistent communication through monthly micro–stewardship meetings. It's a behind-the-scenes look at how strong collaboration turns into better patient outcomes.   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Support the podcast: Venmo Buy me a Ko-fi  

The Infectious Diseases Society of America (IDSA) has released new guidelines for the treatment of complicated urinary tract infections. In this episode, we break down the key updates: Changes to the definition of complicated UTIs The role of newer antibiotic agents How and when to use antibiograms A four-step framework for guiding therapy When carbapenems are necessary—and when other options may be just as effective Shorter durations of therapy Join Dr. Barbara Trautner, Dr. Nicolas Cortés-Penfield, and Dr. Kalpana Gupta as they discuss what these guidelines mean for clinicians, microbiologists, and patients. 👉 Stay tuned until the end for a special announcement.   Resources from this episode: 2025 cUTI IDSA guidelines   Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Support the podcast: Venmo Buy me a Ko-fi  

Bloodstream infections can progress rapidly to sepsis—bringing high costs, high mortality, and lasting effects. In the second episode of this series in collaboration with bioMérieux, Luis speaks with Dr. Jeff Cies, Senior Medical Advisor at bioMérieux, about how molecular diagnostics are reshaping bloodstream infection diagnosis. They discuss: Limitations of traditional blood cultures How rapid molecular tests deliver results faster than ever The impact on outcomes, costs, and antimicrobial resistance The role of stewardship programs in maximizing results What the future holds: direct-from-blood testing, nanotechnology, and AI A powerful look at how innovation in microbiology is changing the fight against sepsis and saving lives. This episode is sponsored by bioMérieux, a global innovator in infectious disease diagnostics. Resources from this episode: bioMérieux Sepsis Webinar Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Support the podcast: Venmo Buy me a Ko-fi  

Brucella is a familiar name in the lab—but recent updates to the sentinel guidelines are reshaping how we approach this pathogen. In this episode, I'm joined by Dr. Laura Filkins and Dr. Paige Larkin to discuss the latest changes: updates in nomenclature, the removal of certain Brucella species from the select agent list, and the renewed emphasis on safety in the lab. We dive into taxonomy controversies, clinical features of brucellosis, and practical steps laboratorians can take to protect themselves while delivering accurate results. Whether you're a student, laboratorian, or clinician, this conversation highlights what you need to know about the evolving landscape of Brucella diagnostics and safety.   Resources from this episode: CDC's Brucellosis Information Page ASM Updates on Brucella Stay connected with Let's Talk Micro: Website: letstalkmicro.com Questions or feedback? Email me at [email protected] Support the podcast: Venmo Buy me a Ko-fi  

Antimicrobial resistance (AMR) continues to grow as a global health threat, making infections harder to treat and leaving fewer options for patients. The need for new antibiotics is an urgent matter — but traditional discovery methods are slow and limited. In this episode of Let's Talk Micro, Luis is joined by Dr. James Collins, professor at MIT and researcher at the Broad Institute, who is leading efforts to apply AI to antibiotic discovery. We discuss how his team used deep learning to uncover promising new compounds, including NG1 and DN1, which showed activity against drug-resistant Neisseria gonorrhoeae and Staphylococcus aureus. We also talk about the challenges of bringing discoveries from computer models to the clinic, and what this approach could mean for the future of the fight against superbugs.   Link to study: https://www.cell.com/cell/abstract/S0092-8674%2825%2900855-4   Check out the website: https://www.letstalkmicro.com/ Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

Last week, we discussed early growth isolates for antimicrobial susceptibility testing (AST) and the importance of obtaining results sooner. This week, we revisit an earlier study that looked at early growth but using disk diffusion. In this episode, I was joined by Dr. Carey-Ann Burnham, Dr. Daniel Webber, and Meghan Wallace to discuss their work. Tune in to hear how this approach could help deliver AST results faster, the challenges of adopting early growth methods, and what it means for clinical microbiology labs. This episode was originally released in 2022 (Episode 39: "Talking disk diffusion performed on early growth").   Link to disk diffusion study: https://doi.org/10.1128/jcm.03007-20   Check out the website: https://www.letstalkmicro.com/ Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

In this episode of Let's Talk Micro, Dr. Jennifer Dien Bard and Dr. Lucas Osborn discuss their recent study on early growth antimicrobial susceptibility testing (AST). Traditional AST often requires 18–24 hours of incubation of isolates, delaying targeted treatment. Their research shows that using 6-hour early growth isolates with both automated and manual methods delivers accurate results up to 18 hours faster—without added costs or specialized equipment. The conversation explores the study design, agreement rates with standard methods, and what faster AST means for patient care and antimicrobial stewardship.  Link to study: https://doi.org/10.1128/jcm.00236-25 Link to disk diffusion study: https://doi.org/10.1128/jcm.03007-20   Check out the website: https://www.letstalkmicro.com/ Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

This week, Luis is joined by Dr. Ashlan Kunz-Coyne to discuss some of the newest antibiotics for urinary tract infections (UTIs). They explore why extended-spectrum beta-lactamases (ESBLs) are a growing concern, the impact of E. coli sequence type 131, and where new agents like cefepime–enmetazobactam, sulopenem, and tebipenem fit into treatment. The conversation also highlights clinical data, stewardship considerations, and how the infectious diseases and microbiology communities can work together to preserve these options. Plus, Dr. Kunz-Coyne reveals her favorite bug—Stenotrophomonas maltophilia—and why it's unlike any other.    Link to sulopenem episode: https://directory.libsyn.com/episode/index/id/35868135   Check out the website: https://www.letstalkmicro.com/ Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

Season 5 premieres today!  Antimicrobial susceptibility testing (AST) is essential for guiding effective treatment—but how has it evolved, and what challenges are we still facing? In the first episode of this series in collaboration with bioMérieux, Dr. Andrea Prinzi joins Let's Talk Micro to explore the evolution of AST—from early methods to modern platforms like VITEK 2. We discuss the known issue of outdated breakpoints in clinical practice, the challenges with regulatory hurdles, and the impact of the 21st Century Cures Act. What is the Cures Act, and how does it affect the way breakpoints are adopted? Why are delays still happening? And most importantly—what can laboratories, organizations, and manufacturers do to drive change? Tune in to understand where we've been, where we are, and where we need to go in the world of AST.   This episode is sponsored by bioMérieux, a global innovator in infectious disease diagnostics.   Resources from bioMérieux: https://info.biomerieux.com/clin_us_2024_ams_handbook?utm_source=podcast&utm_medium=show-notes&utm_campaign=LetsTalkMicro Additional breakpoint links: https://doi.org/10.1128/jcm.00106-25   Check out the website: https://www.letstalkmicro.com/ Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro

This episode of Let's Talk Micro features Dr. Andrea Prinzi, a clinical microbiologist and frequent contributor to the American Society for Microbiology. Andrea joins the podcast to discuss a critical topic in clinical microbiology: antimicrobial susceptibility testing and breakpoints. Are labs using outdated breakpoints—and what does that mean for patient care? How are breakpoints determined and updated? Dr. Prinzi breaks it all down and shares insights from her article on the subject. Tune in to learn why staying current on breakpoints matters and what steps labs can take to improve antimicrobial stewardship. This episode was originally published on May 5th, 2022.   Check out the website: https://www.letstalkmicro.com/ Link to articles: https://asm.org/Articles/2022/February/Updating-Breakpoints-in-Antimicrobial-Susceptibili https://doi.org/10.1128/jcm.00106-25   Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

The Clinical Microbiology Procedures Handbook (CMPH) has long been a trusted resource for laboratories.  With the release of its 5th edition, this essential reference continues to guide clinical microbiologists through evolving standards, methods, and best practices. In this episode, Luis is joined by the editors‑in‑chief, Dr. Amy Leber and Dr. Carey‑Ann Burnham, to explore what's new in the latest edition, how the CMPH supports day‑to‑day work in the lab, and why it remains a cornerstone in clinical microbiology. (Originally published May 4, 2023.) Check out the website: https://www.letstalkmicro.com/ Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

This week, Luis is joined by Dr. Christopher Doern from VCU Health to explore the complex world of diabetic wound cultures. How does diabetes impair wound healing—and what makes these infections so difficult to diagnose and treat? Dr. Doern dives into the microbiology behind diabetic foot wounds, highlighting the impact of bacterial diversity, specimen quality, and the limitations of traditional culture methods. He also shares best practices for workup strategies and antibiotic selection in these often polymicrobial infections. Link to studies mentioned in the episode: https://pubmed.ncbi.nlm.nih.gov/28669650/ https://doi.org/10.1111/j.1742-481X.2011.00937.x https://academic.oup.com/ofid/article/10/10/ofad495/7292955   Want to be added to Dr. Doern's email list? Reach out to [email protected]   Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

Recorded live at ASM Microbe 2025, this episode captures the energy of the American Society for Microbiology's annual event. Luis shares his top takeaways from the conference floor—including standout poster presentations, emerging technologies, and trending topics in microbiology. One clear theme dominated this year: rapid diagnostics for identification and susceptibility testing. Tune in for a front-row seat to the latest in clinical microbiology innovation.   Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro  

In this episode of Let's Talk Micro, we dive into the fast-paced world of rapid antimicrobial susceptibility testing (AST). Recorded live from ASM Microbe 2025, Luis is joined by Dr. Carey-Ann Burnham, CEO of Pattern Bioscience and Editor-in-Chief of the ASM Case Reports journal. Together, they explore how innovative technologies—like single-cell microbiology—are transforming clinical microbiology and accelerating diagnostic results. What is single-cell microbiology, and how does it improve rapid diagnostics? They also discuss case reports from ASM that highlight the real-world impact of timely diagnostic tools on patient care. It's where speed meets science—and the stories behind the data truly matter. Questions? Feedback? Send those to [email protected] Want to support the podcast? Here's how: Venmo: https://venmo.com/u/letstalkmicro Buy me a Ko-fi: https://ko-fi.com/letstalkmicro