Mentors at Your Benchside cover art

All Episodes

Mentors at Your Benchside — 115 episodes

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Title
1

Hepatocyte Counting Methods: From Manual Counts to Fast, Accurate Automation

2

Single Cell Sequencing: Tips to Optimize Nuclei Extraction and Counting

3

Personal Advice on How to Respond to Criticism of Your Grant Proposal Reviews

4

Isoelectric Focusing: A Simple Way to Enhance Your Protein Separation

5

Top 10 Uses of Microbes in Biotechnology

6

Let’s Talk About Stats: Methods for Comparing Two Sets of Data

7

Personal Advice on Building Your Professional Network. It Takes a Village

8

4 Fixatives for Histology and Cytometry: Perfect Your Preservation

9

Microscope Disinfection: A Quick Guide

10

Tissue Processing For Histology: What Exactly Happens?

11

Multiple Fragment Ligation: The Why and How

12

Funding Opportunities and the Flow of Money in Science

13

Maxam–Gilbert Sequencing: What It Is and 3 Modern Applications

14

Confocal Laser Scanning Microscopy Explained In 3 Easy Steps

15

How to Become an Expert at Getting Funded

16

5 Types of Difficult Lab Supervisor and How to Handle Them

17

7 Top Tips to Make the Most of Your Flow Cytometry Training

18

What Reagents Can You Use Past Their Chemical Expiry Date?

19

How to Write an Effective Research Interest Statement

20

How to Preserve Microorganisms: Store Your Cells Better

21

Overhang PCR: Add Missing DNA Sequences Using Primers

22

Practical Applications and Considerations of Phenol-Chloroform Extraction

23

How to Become a Bioinformatician

24

8 Cell Lysis Methods to Break Cell Walls

25

Genetic Variants Explained

26

How to Identify Supercoils, Nicks and Circles in DNA Plasmid Preps

27

Simplicity in Science: How to Increase your Research Effectiveness by Doing Less

28

How to Totally Nail Your First in situ Hybridization

29

Choosing The Right Blood Collection Tubes

30

A Step-by-Step Guide to Designing qPCR Primers

31

How to Passage Cells in Culture

32

3 Easy Tips for Avoiding Measurement Drift in Analytical Balances

33

How to Make Your Own Chemically Competent Cells

34

5 Easy Tips for Keeping Your Centrifuge Alive

35

How Blunt-End Cloning Works

36

A Beginner’s Guide to Hematoxylin and Eosin Staining

37

5 Ingredients for the Perfect Protein Purification Buffer

38

How FRET Works: A Guide to Visualizing Protein Interactions

39

How Can We Make Science More Accessible?

40

A Short History of Cell Biology

41

How to use a Hemocytometer

42

Understand How Alkaline Lysis Works

43

All About the qPCR Standard Curve: The Key to Good PCR Data

44

10 Fun Hobbies for Scientists

45

How Histology Slides Are Prepared

46

6 Laboratory Sterilization Methods and How They Work

47

Types of Digital Images for Stunning Science Publications

48

5 Controls for Immunofluorescence: A Beginner’s Guide

49

What is Water of Crystallization? Everything You Need to Know

50

Better Plasmid Purification: 11 Reasons Your Plasmid Yield is Low

51

The Importance of Delegation for Scientists

52

How To Make ECL Reagent: 4 Ingredients, Better Blots

53

3 Hot Tips to Optimize Your Western Blot Transfers

54

Top 5 of the Most Commonly Used Cell Lines

55

Get a Grip: Dealing with Sweaty Glove Hands

56

What Ponceau S Staining Can Reveal About Your Western Blot

57

Plasmid Incompatibility: Should You Care?

58

Protein Colocalization: 2 Essential Methods to Prove Protein Overlap

59

Why Do Enzymes Have Optimum Temperatures?

60

The How and Why of Removing Serum From Your Media

61

Cell Passage Number Explained

62

10 Common PPE Sins

63

PPE for Eye and Face Protection—Better Safe than Sorry!

64

How to Handle Strong Acids in the Lab

65

How to Use a pH Meter Correctly in 4 Simple Steps

66

The Dangers of UV Light in the Lab: Keep You and Your Experiments Safe

67

Problems Amplifying GC-rich regions? 5 Easy Solutions

68

8 Steps to More Successful Experiments

69

How Buffers Work—A Guide For Biologists

70

What Is a Ct Value?

71

Different DNA Polymerases Explained

72

10 Good and Evil Ways to Use Your Dr Title

73

How DNA Extraction Kits Work in the Lab

74

Funny Fruit Fly Gene Names

75

How to clean and calibrate your lab balance

76

How to Write a Thoughtful Discussion for Your Scientific Paper

77

How Using Oil Immersion Microscopy Can Increase Your Resolution

78

Artificial Intelligence in Biology

79

Does Your h-index Measure Up?

80

How to Check the Accuracy of Your Pipette in 7 Easy Steps

81

How to Make the Perfect Agar Plate Every Time

82

Top 5 Protein Quantification Assays

83

Top 10 Worst Lab Smells

84

Is Your Bacterial Culture Still Growing? A Primer on OD­600 Measurements

85

How CRISPR-Cas13 Can Revolutionize Your RNA Research

86

Keep Calm and Spin On: The Whats and Whys of Centrifugation

87

Protease Inhibitors 101: How They Work and How to Use Them

88

Constant Current or Voltage in SDS-PAGE: The Great Debate

89

DNA Ligation: How it Works & 6 Top Tips

90

What Is Cryo-Electron Microscopy? A Brief Introduction

91

The Basics: How Phenol Extraction of DNA Works

92

Postdoc Interview Preparation: Sample Questions and Answers

93

Creativity in Science: How a Good Imagination Can Help Your Research

94

Ten Bad Chemicals in the Lab and What They do to You!

95

How Confluent Are Your Cells? A Beginner’s Guide to Measuring Cell Culture Confluency

96

Laemmli Buffer: What Is It for Anyway?

97

What is Alternative Splicing, and Why Is It Important?

98

Why Is SDS-PAGE Run Vertically? Here are 3 Great Answers

99

How (and Why) To Manipulate Plasmid Copy Number

100

Top 10 Tips for Viva Success

101

A Brief History of CRISPR-Cas9 Genome-Editing Tools

102

How to Measure and Improve Lab Accuracy and Precision

103

The Ultimate Guide to Choosing a Fluorescent Protein

104

DNA Precipitation: Ethanol vs. Isopropanol

105

How Fluorescent Molecules Work: Shine Bright like a Diamond

106

Which Type of Ethanol Should I Use?

107

What the HEK? A Beginner’s Guide to HEK293 Cells

108

How SDS-PAGE Works

109

How a Jellyfish Changed Biology: the Discovery and Development of GFP

110

Ethanol Precipitation of DNA and RNA: How it Works

111

20 Telling Signs You're a Scientist

112

What is PCR? The Beginner’s Guide

113

A Short History of Cryo-Electron Microscopy

114

17 Ways to Stop Pipetting Errors From Ruining Your Experiments

115

Is it Worth Doing a PhD After a Master’s?