1081-微生物原位单细胞组学研究

这项研究介绍了一种名为激光诱导前向转移 (LIFT) 的新技术，用于在自然环境中直接获取高质量的微生物单细胞基因组和转录组。该方法摆脱了对微流控技术的依赖，能够从小鼠肠道、人类唾液及肿瘤切片等复杂样本中精准分离单个细菌。通过结合拉曼光谱和细胞形态学特征，研究人员实现了在不破坏周边环境的情况下对特定微生物进行自动化筛选。实验成功揭示了地衣芽孢杆菌在孢子形成过程中的细胞命运异质性，并分析了大肠癌组织中驻留细菌的代谢活性。这种原位单细胞组学方案为探索宿主与微生物之间的空间相互作用以及鉴定稀有菌群提供了强有力的工具。References:Lan X, Liang Q, He J, et al. Microbial single-cell omics in situ[J]. Cell Genomics, 2026.前往小宇宙评论区与主播互动

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1081-微生物原位单细胞组学研究

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这项研究介绍了一种名为激光诱导前向转移 (LIFT)...

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