114: One-hour extraction-free LAMP HPV test for point-of-care screening

Barra MJ et al et al., Nature Communications (2025) 16:7295 - This study reports development and analytic evaluation of an extraction-free DARQ LAMP assay detecting HPV16, HPV18, and HPV45 plus a cellular control with a <1 hour sample-to-answer workflow on a low-cost benchtop heater/fluorimeter. Clinical testing in Houston (n=38) and Maputo (n=191) showed 100% and 93% concordance, respectively, with the GeneXpert reference test. Key terms: HPV16, HPV18, HPV45, LAMP, point-of-care. Study Highlights:The authors developed a multiplexed DARQ LAMP assay targeting HPV16, HPV18, HPV45 and a cellular control and implemented an extraction-free NaOH lysis allowing direct addition of lysate to the reaction. Limits of detection with extracted DNA were 57, 11.7, and 14.3 copies/reaction for HPV16, HPV18, and HPV45, respectively, and with crude lysate were ~30.4 SiHa, 11.7 HeLa, and <10 MS751 cells/reaction. The assay runs on a portable Axxin T8-ISO device in under one hour and achieved 100% concordance in Houston (n=38) and 93% concordance in Maputo (n=191) versus GeneXpert. Extraction-free workflow simplified specimen handling but contributed to reduced sensitivity for samples with low viral load. Conclusion:An extraction-free, multiplexed DARQ LAMP assay provides rapid (<1 h), sensitive detection of HPV16/18/45 with high concordance to a WHO-prequalified reference test and a simple workflow suitable for point-of-care use in resource-limited settings, while further clinical validation and expansion to additional HPV types are needed. Music:Enjoy the music based on this article at the end of the episode. Article title:One-hour extraction-free loop-mediated isothermal amplification HPV DNA assay for point-of-care testing in Maputo, Mozambique First author:Barra MJ et al Journal:Nature Communications (2025) 16:7295 DOI:10.1038/s41467-025-62454-x Reference:Barra MJ et al. One-hour extraction-free loop-mediated isothermal amplification HPV DNA assay for point-of-care testing in Maputo, Mozambique. Nature Communications (2025) 16:7295. https://doi.org/10.1038/s41467-025-62454-x License:This episode is based on an open-access article published under the Creative Commons Attribution 4.0 International License (CC BY 4.0) – https://creativecommons.org/licenses/by/4.0/ Support:Base by Base – Stripe donations: https://donate.stripe.com/7sY4gz71B2sN3RWac5gEg00 Official website https://basebybase.com On PaperCast Base by Base you'll discover the latest in genomics, functional genomics, structural genomics, and proteomics. Episode link: https://basebybase.com/episodes/one-hour-extraction-free-loop-mediated-isothermal-amplification-hpv-dna-assay-for-point-of-care-testing-in-maputo-mozambique QC:This episode was checked against the original article PDF and publication metadata for the episode release published on 2025-08-22. QC Scope:- article metadata and core scientific claims from the narration- excludes analogies, intro/outro, and music- transcript coverage: Audited the transcript’s depiction of the DARQ LAMP extraction-free HPV assay, its workflow, device, clinical evaluations, and performance metrics.- transcript topics: HPV biology and cervical cancer screening context; DARQ LAMP methodology and extraction-free lysate; Targets HPV16/HPV18/HPV45 and cellular control; Point-of-care device and workflow (65 C, T8-ISO); Clinical evaluation in Houston and Maputo; Performance metrics, concordance, and limitations QC Summary:- factual score: 10/10- metadata score: 10/10- supported core claims: 8- claims flagged for review: 0- metadata checks passed: 4- metadata issues found: 0 Metadata Audited:- article_doi- article_title- article_journal- license Factual Item...