1170-D&D-seq: Single-Cell DNA-Protein Interactions

Researchers have developed D&D-seq, a novel single-cell technology designed to map DNA-protein interactions, even those that are weak or transient. By fusing a cytosine base editor to a nanobody, the method induces specific C-to-U mutations at sites where proteins like transcription factors are bound to the genome. This technique addresses the limitations of older methods that struggled with low-input samples or high-salt conditions that disrupted fragile molecular bonds. D&D-seq is highly versatile, integrating seamlessly with multi-omic workflows like scATAC-seq to co-profile chromatin accessibility and protein binding in individual cells. In practical applications, the authors used the tool to identify disrupted CTCF binding in primary mutant T cells and to predict 3D chromatin structures. Ultimately, this approach provides a high-resolution, direct link between a cell's genotype and its regulatory landscape.References:Chi WY, Yoon SH, Goksel E, Mekerishvili L, Pelt J, Lin Y, Prieto T, Zinno J, Ganesan S, Potenski C, Izzo F, Landau DA, Raimondi I. Single-cell mapping of regulatory DNA-protein interactions. Cell. 2026 Jun 4:S0092-8674(26)00573-8. doi: 10.1016/j.cell.2026.05.014. Epub ahead of print. PMID: 42242226.前往小宇宙评论区与主播互动