1195-Spatial Pharmacobiology of Antibody Delivery

The paper introduces Single-Cell Spatial Pharmacobiology (SSP), a novel analytical framework designed to visualize and measure how therapeutic antibodies interact with human solid tumors. By combining fluorescently labeled drug imaging with multiplexed spatial proteomics, researchers can now observe drug distribution and target engagement at cellular resolution rather than relying on low-resolution systemic scans. Investigations into patients with head and neck and pancreatic cancers revealed that drug delivery is significantly hindered by specific tumor microenvironment (TME) structures. Specifically, the study identified fibroblast-activation-protein-positive (FAP+) cells and periostin-rich extracellular matrix assemblies as conserved physical barriers that limit antibody penetration. These findings suggest that SSP can identify spatial biomarkers and resistance mechanisms, ultimately helping to optimize dosing strategies and improve the efficacy of precision oncology.References:Lu G, Hickey J W, Haist M, et al. Single-cell spatial pharmacobiology identifies conserved stromal barriers to therapeutic antibody delivery in human solid tumors[J]. Nature Biotechnology, 2026: 1-15.前往小宇宙评论区与主播互动