1226-VIPerturb-seq: Genome-Wide Single-Cell CRISPR Screening

The paper introduce VIPerturb-seq, a versatile platform designed to make genome-wide single-cell CRISPR screens more efficient and affordable. By utilizing a synthetic split-barcode system and probe-based detection, the method bypasses the high costs and technical hurdles of traditional sequencing workflows. This approach supports fixed-cell profiling, allowing researchers to specifically isolate and sequence cells exhibiting target phenotypes, such as high protein levels, prior to analysis. Additionally, the platform integrates combinatorial indexing to enhance experimental throughput by up to 50-fold while maintaining high data sensitivity. Through the GuEST-List library, the study successfully identifies novel regulators of the protein vimentin, demonstrating the tool's power for discovery. Ultimately, VIPerturb-seq provides a scalable framework for individual laboratories to systematically map gene functions across the entire human genome.References:Bradu A, Blair J D, Grabski I N, et al. Genome-wide single-cell perturbation screens with VIPerturb-seq[J]. bioRxiv, 2026.前往小宇宙评论区与主播互动