1372-Whole-Cell Digital Twin Simulations of Organelle

Researchers have developed a whole-cell digital twin framework that bridges the gap between live-cell imaging and computational simulation to model intracellular organelle dynamics. By integrating 4D lattice light-sheet microscopy with particle-based simulations, the study creates spatially accurate models of mitochondrial networks and their transport along microtubules. These digital twins successfully predict how mitochondria react to cytoskeletal disruptions and stress without requiring the recalibration of parameters. A key discovery reveals that microtubule topology acts as a structural gate, meaning that motor activity alone cannot drive mitochondrial clustering if the network connectivity is insufficient. This platform provides a biologically grounded method for investigating complex cellular behaviors and predicting the effects of pharmacological perturbations.References:Arkfeld E, Wang Z, Hakozaki H, et al. Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data[J]. bioRxiv, 2025: 2025.04. 09.647865.前往小宇宙评论区与主播互动