198: Mechanical Confinement and the Shape-Shifting Life of Melanoma Cells

Mechanical Confinement and the Shape-Shifting Life of Melanoma Cells Music:Enjoy the music based on this article at the end of the episode. License:This episode is based on an open-access article published under the Creative Commons Attribution 4.0 International License (CC BY 4.0) – https://creativecommons.org/licenses/by/4.0/ Support:Base by Base – Stripe donations: https://donate.stripe.com/7sY4gz71B2sN3RWac5gEg00 Official website https://basebybase.com On PaperCast Base by Base you’ll discover the latest in genomics, functional genomics, structural genomics, and proteomics. Episode link: https://basebybase.com/episodes/mechanical-confinement-and-the-shape-shifting-life-of-melanoma-cells ️ Episode:198: Mechanical Confinement and the Shape-Shifting Life of Melanoma Cells ️ Season:1 Article title:Mechanical confinement governs phenotypic plasticity in melanoma Journal:Nature DOI:10.1038/s41586-025-09445-6 QC:This episode was checked against the original article PDF and publication metadata for the episode release published on 2025-11-14. QC Scope:- article metadata and core scientific claims from the narration- excludes analogies, intro/outro, and music- transcript coverage: Audited core scientific claims and themes described in the transcript: confinement-driven phenotype switching (go-or-grow), HMGB2-mediated chromatin remodeling, cytoskeletal (acetylated tubulin cage) remodeling, Nesprin2/LINC association, Notch/BRN2 axis in invasion, and drug-tolerance implications.- transcript topics: Confinement-driven phenotype switching (go-or-grow); Neuronal invasion gene program and MITF suppression; HMGB2 upregulation and chromatin remodeling; Perinuclear acetylated tubulin cage and ATAT1 involvement; Nesprin2 (SYNE2) and LINC complex in HMGB2 enrichment; Nuclear stiffening via Lamin A/C QC Summary:- factual score: 10/10- metadata score: 10/10- supported core claims: 8- claims flagged for review: 0- metadata checks passed: 4- metadata issues found: 0 Metadata Audited:- article_doi- article_title- article_journal- license Factual Items Audited:- Confinement induces neuronal invasion program and MITF downregulation (SOX11, NEUROD1, NNAT, NEFM upregulation; MITF downregulation)- HMGB2 upregulation is induced by confinement and HMGB2 concentration increases (approximately doubles) under confinement- Confinement triggers assembly of a perinuclear acetylated tubulin cage; ATAT1 mediates tubulin acetylation- Nesprin2 (SYNE2) is required for HMGB2 enrichment and for perinuclear cage assembly- HMGB2 increases chromatin accessibility at neural crest/neuronal loci; AP-1 and SOX9 motifs enriched- Notch signaling and BRN2 axis mediate HMGB2-driven invasion QC result: Pass.