205: Ancient RNA Expression Profiles from the Woolly Mammoth

Ancient RNA Expression Profiles from the Woolly Mammoth Music:Enjoy the music based on this article at the end of the episode. License:This episode is based on an open-access article published under the Creative Commons Attribution 4.0 International License (CC BY 4.0) – https://creativecommons.org/licenses/by/4.0/ Support:Base by Base – Stripe donations: https://donate.stripe.com/7sY4gz71B2sN3RWac5gEg00 Official website https://basebybase.com On PaperCast Base by Base you’ll discover the latest in genomics, functional genomics, structural genomics, and proteomics. Episode link: https://basebybase.com/episodes/ancient-rna-expression-profiles-from-the-woolly-mammoth ️ Episode:205: Ancient RNA Expression Profiles from the Woolly Mammoth ️ Season:1 Article title:Ancient RNA Expression Profiles from the Woolly Mammoth Journal:Cell DOI:10.1016/j.cell.2025.10.025 QC:This episode was checked against the original article PDF and publication metadata for the episode release published on 2025-11-21. QC Scope:- article metadata and core scientific claims from the narration- excludes analogies, intro/outro, and music- transcript coverage: Audited the transcript's coverage of the article’s main experimental design, data-quality checks, major results, tissue-level interpretation, and preservation rationale, including sample collection/preservation, RNA sequencing and fragment mapping, damage signatures, exonic reads, tissue identity, sex determination, an- transcript topics: Sample collection and permafrost preservation; RNA sequencing, fragment length, and mapping strategy; Damage signatures and data authenticity (CU deamination, 5' end bias); Exonic mapping and exon-exon junctions indicating mature mRNA; Tissue identity and metabolism (skeletal muscle; slow-twitch markers MYH7, TNNT1, NEB); Genetic sex determination (Y-chromosome transcripts USP9Y) and autosomal DNA QC Summary:- factual score: 10/10- metadata score: 10/10- supported core claims: 7- claims flagged for review: 0- metadata checks passed: 4- metadata issues found: 0 Metadata Audited:- article_doi- article_title- article_journal- license Factual Items Audited:- RNA recovered from soft tissues of woolly mammoths; 3 of 10 samples showed endogenous mammoth material- RNA fragments map to exonic regions and span exon-exon junctions, indicating mature mRNA- Tissue expression profile matches skeletal muscle with slow-twitch fiber markers (MYH7, TNNT1, NEB)- Genetic sex determined as male via Y-chromosome transcripts such as USP9Y- Two novel mammoth-specific microRNAs predicted (MPR novel 4 and MPR novel 5) in Afrotherians- Mir-1 variant with GG unpaired bulge observed, shared among Proboscidians QC result: Pass.