289: MinION detection of chimeric reads in murine Ifna/Ifnb amplicons and ligation-related artifact prevalence

White R et al., F1000Research - Investigation of chimeric reads in MinION nanopore sequencing of short PCR amplicons, focusing on ligation-related artifacts, barcode tracing, and the prevalence of cross-gene chimeras in murine Ifna/Ifnb sequencing runs. Music:Enjoy the music based on this article at the end of the episode. Article title:Investigation of chimeric reads using the MinION First author:White R Journal:F1000Research DOI:10.12688/f1000research.11547.2 Reference:White R, Pellefigues C, Ronchese F, Lamiable O, Eccles D. Investigation of chimeric reads using the MinION [version 2; peer review: 2 approved]. F1000Research. 2017;6:631. https://doi.org/10.12688/f1000research.11547.2 License:This episode is based on an open-access article published under the Creative Commons Attribution 4.0 International License (CC BY 4.0) – https://creativecommons.org/licenses/by/4.0/ Support:Base by Base – Stripe donations: https://donate.stripe.com/7sY4gz71B2sN3RWac5gEg00 Official website https://basebybase.com On PaperCast Base by Base you’ll discover the latest in genomics, functional genomics, structural genomics, and proteomics. QC:This episode was reviewed as a special editorial release. QC Scope:- human-authored narration, music, and publication metadata- excludes automated single-article AI QC because this episode was not produced by the standard AI narration pipeline- source attribution and descriptive metadata were reviewed manually QC Summary:- production mode: human-written and human-produced episode- automated transcript-vs-paper AI QC: not applicable- metadata, source attribution, and release assets were reviewed manually Metadata Audited:- article_doi- article_title- article_journal- reference- license Factual Items Audited:- episode classified as a human-produced release- canonical human narration retained as the source of record- canonical human-produced music retained as the source of record- automated NotebookLM regeneration intentionally not used for this episode QC result: Editorial exception. This episode was approved after manual review.