Behind the Vial; Freeze Drying 101; Why Lyophilisation works and what is Sublimation

This episode explains the lyophilisation process used in peptide manufacturing, showing how liquid formulations are freeze dried to improve stability. It covers the key stages of freezing, primary drying, secondary drying, and final stoppering, and explains how water is removed by sublimation under vacuum. Using real factory footage from Aaron’s Biochemapi facility, the episode walks through what happens inside the freeze dryer and shows how partially stoppered vials become sealed, freeze-dried products.