E6: Illumina Sequencing Part II

Glued DNA bridges and rainbow clustersChapters:00:00-Flowcells03:40-DNA glued to glass09:00-Bridge amplification15:50-Cluster density and patterned Flowcells28:30-Illumina SBS summary30:30-Limits33:00-Multiplexing and Demultiplexing- enseqlopedia on patterned vs unpatterned flowcell:http://enseqlopedia.com/2016/01/almost-everything-you-wanted-to-know-about-illumina-hiseq-4000-and-some-stuff-you-didnt/- ClevaLabs on Illumina sequencing: https://www.youtube.com/watch?v=WKAUtJQ69n8- Yourgenome on how the Illumina workflow works:https://www.yourgenome.org/facts/what-is-the-illumina-method-of-dna-sequencing/- DNATech comparing Illumina Machines:https://dnatech.genomecenter.ucdavis.edu/illumina-high-throughput-sequencing/- iBiology on sample preparation for Illumina sequencing:https://www.youtube.com/watch?v=PFwSe09dJX0- StatQuest on how to use Illumina sequencing for RNA analysis:https://www.youtube.com/watch?v=tlf6wYJrwKY- Illumina Flowcell and sequencing machines pictures:https://www.illumina.com/company/news-center/multimedia-images.html- Nice explainer about paired-end sequencing:https://www.youtube.com/watch?v=gVrmfLXJRGMSince 2018, West German Genome Center (https://wggc.de/)operates as the next generation sequencing facility and supportsresearch in genomics & transcriptomics by providing expertise andsequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/)- network of NGS core facilities in Germany. Listen to our podcast and send your feedback to ( podcast[dot]explain[at]gmail[dot]com ).The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).Our podcast is an outreach activity within the national genomics facility "West German Genome Center" funded by the DFG (https://www.dfg.de/) (No. 407493903).Clarification: Each Flowcell on the Illumina platform has 1-8channels, each channel can be filled with multiple different samples- DRLicense NoticeThis podcast including all episodes, unless otherwise noted, is licensed under a ⁠⁠⁠Creative Commons Attribution 4.0 International License⁠⁠⁠.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.Attribution:Podcast Title: Explain PodcastCreators: Iuliia Novoselova, Daniel RickertWebsite: ⁠⁠⁠https://ngs-cn.de/explain-podcast/⁠