E8: Nanopore

Pushing spaghetti through holesChapers:00:00 Intro11:15 Measuring Ions17:00 Quality23:00 Duplex24:30 MinION25:15 PromethION28:20 Methylation29:00 Direct RNA sequencing38:25 Basecalling46:00 Basecalling bias53:50 Sequencing proteins- Nanopore explainer: https://nanoporetech.com/applications/dna-nanopore-sequencing- Oxsci explaining the technology:https://oxsci.org/pore-over-this-advances-in-dna-sequencing/- History of Nanopore:https://www.whatisbiotechnology.org/index.php/science/summary/nanopore/nanopore-sequencing-makes-it-possible-to-decode-the- Shmou’s Biology explaining Nanopore sequencing:https://www.youtube.com/watch?v=MlluAjhzXqI- A direct comparison of Nanopore and Illumina output:https://medresearch.umich.edu/office-research/about-office-research/biomedical-research-core-facilities/advanced-genomics-core/technologies/next-generation-sequencing- A nice Nanopore QC tool we use: https://hpc.nih.gov/apps/pycoQC.html- How a MinION looks like:https://www.whatech.com/og/markets-research/medical/658327-global-nanopore-sequencing-market-2020Since 2018, West German Genome Center (https://wggc.de/)operates as the next generation sequencing facility and supportsresearch in genomics & transcriptomics by providing expertise andsequencing services. WGGC is a part of the Next Generation Sequencing Competence Network (NGS-CN: https://ngs-kn.de/)- network of NGS core facilities in Germany.Listen to our podcast and we're happy to receive your feedback&questions via Email ( podcast[dot]explain[at]gmail[dot]com ).The Explain Podcast is recorded at the Medienlabor at Heinrich-Heine University of Düsseldorf (https://medienlab.phil.hhu.de/).Our podcast is an outreach activity within the national genomicsfacility "West German Genome Center" funded by the DFG(https://www.dfg.de/) (No. 407493903).License NoticeThis podcast including all episodes, unless otherwise noted, is licensed under a ⁠⁠⁠Creative Commons Attribution 4.0 International License⁠⁠⁠.You are free to share, remix, and use this content as long as you give appropriate credit, provide a link to the license, and indicate if changes were made.Attribution:Podcast Title: Explain PodcastCreators: Iuliia Novoselova, Daniel RickertWebsite: ⁠⁠⁠https://ngs-cn.de/explain-podcast/⁠