Episode 27 - In The Lab: Electrophoresis, Immunoelectrophoresis and Serum Free Light Chain Assays episode artwork

EPISODE · Dec 10, 2024 · 48 MIN

Episode 27 - In The Lab: Electrophoresis, Immunoelectrophoresis and Serum Free Light Chain Assays

from Bloody Minded · host Bloody Minded Crew

Thought you were free of myeloma acronyms? Don’t be ridiculous - this is the haematology lab! We were born in the acronyms, moulded by them. We didn’t see a complete word until advanced training and even then it was nothing but BLINDING. Your lab tests betray you because they belong to us… but in this episode we’ll let you in on some secrets. Join Nick, Cale and Bashar in the haematology (and closely adjacent immunology) lab as they chat about just how we do that electrophoresis, why an iEPG is different to an EPG and just how useful Bence-Jones proteins are(n’t).Timestamps3:15 - Bone marrows - not as bad as they sound?4:25 - What even do we do with the marrow?10:57 - Eponymous plasma cells with weird morphology13:04 - EPG - Electrophoresis22:23 - IEPG - Immunoelectrophoresis29:09 - What's the deal with Bence Jones proteins33:27 - SFLC Measurements37:07 - The Hook/Prozone Effect40:55 - Light chain measurement in renal dysfunction44:32 - Take Home MessagesSupport the showCheck out show notes, additional references and (sometimes) extra content at www.bloodyminded.com.au

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Thought you were free of myeloma acronyms? Don’t be ridiculous - this is the haematology lab! We were born in the acronyms, moulded by them. We didn’t see a complete word until advanced training and even then it was nothing but BLINDING. Your lab tests betray you because they belong to us… but in this episode we’ll let you in on some secrets. Join Nick, Cale and Bashar in the haematology (and closely adjacent immunology) lab as they chat about just how we do that electrophoresis, why an iEPG...

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Episode 27 - In The Lab: Electrophoresis, Immunoelectrophoresis and Serum Free Light Chain Assays

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