How PCR, CRISPR, and animal models are advancing oncology research

Cancer research is moving fast, and molecular tools are helping scientists see more, measure more, and ask better questions. In this Science Snapshot episode of Absolute Gene-ius, Jordan Ruggieri and Lisa Crawford revisit memorable oncology-related clips from past conversations. Dr. Raquel Munoz explains how CAR-T cells are engineered to recognize and kill cancer cells, and how PCR-based methods can help confirm successful modification and track CAR-T expansion. Dr. Dustin Rubinstein shares how CRISPR and genome editing are being used to create pig models, including “onco-pig,” that may better reflect aspects of human cancer biology than traditional models. The episode also connects cell-free DNA research from Dr. Lee Ann Baxter-Lowe to oncology applications, before turning to Dr. Kathie Sollweck’s discussion of circulating tumor DNA and the sensitivity needed to detect low mutational burden in liquid biopsy research. Finally, Valeria Rangel offers practical perspective on multiplex PCR assay design. And because it is Absolute Gene-ius, the science comes with some laughs, a few pig jokes, and a reminder that even complex oncology methods can be surprisingly fun to talk about. Visit the Absolute Gene-ius page to learn more about the guests, hosts, and science featured in the series. Explore how Applied Biosystems™ QuantStudio™ Digital and Real-Time PCR Systems support research across neuroscience, oncology, agriculture, and more.