Investigation of the Pathomechanism of Chronic Cough Using an In Vitro Approach

Keywords Chronic cough, TRP receptors, P2X3 receptors, neuroplasticity, allergy, immunology, ATP release, bronchial epithelial cells, therapeutic approaches, research     Summary: In this episode of the Clinical and Experimental Allergy podcast, Dr. Jonathan Bernstein discusses his research on chronic cough, focusing on the role of TRP receptors and P2X3 receptors in understanding the mechanisms behind this condition. He shares insights from his in vitro studies, unexpected findings related to chronic cough pathways, and potential future therapeutic approaches targeting these receptors.   Takeaways Dr. Bernstein is a professor of medicine at the University of Cincinnati. His research focuses on chronic cough and its underlying mechanisms. In vitro assays help isolate different cell activation pathways. ATP release is critical for activating P2X3 receptors on bronchial epithelial cells. Unexpected pathways activated by ATP include FAC signaling and IL-4. P2X3 antagonists can down-regulate pathways promoting airway inflammation. TRP receptors are the first interface between the body and the environment. Nonspecific triggers can activate TRP receptors, leading to increased ATP release. Selective antagonists for TRP receptors are still lacking. The nervous system plays a critical role in cough reflex mechanisms.