NMDA Receptor Protocols (Methods in Molecular Biology)

Detailed laboratory protocols and methodological descriptions focused heavily on studying the NMDA receptor, a type of ligand-gated ion channel. Several articles describe the preparation and analysis of the receptor using cellular expression systems, including Xenopus oocytes, HEK 293 cells, and CHO cells, for both functional and biochemical characterization. Additionally, extensive procedures are outlined for electrophysiology techniques, such as two-electrode voltage-clamp and patch-clamp, as well as ligand-binding assays utilizing radiolabeled probes like $\text{[}^{125}\text{I]}-\text{MK-801}$. The sources also cover advanced methods like immunocytochemistry, viral vector expression systems (baculovirus and herpesvirus), and the in vitro selection of peptide ligands using phage-displayed libraries, collectively providing a comprehensive guide to NMDA receptor research.You can listen and download our episodes for free on more than 10 different platforms:https://linktr.ee/medicine_made_simpleProduced by:https://www.podcaistudio.com/