Unlocking Lung Fibrosis Insights with FFPE Tissue Samples | Spatial Transcriptomics Reveals RNA Potential

In this episode of SciBud, your go-to podcast for the latest in science, host Maple delves into an intriguing study that harnesses the power of spatial transcriptomics to tackle the challenge of lung fibrosis. Using archived formalin-fixed and paraffin-embedded (FFPE) tissue samples, researchers explored gene expression in mouse models treated with the drug bleomycin, revealing a striking overlap of over 90% with data from fresh tissues. Maple breaks down how this innovative technique can illuminate the mechanisms behind fibrosis—characterized by tissue thickening and scarring—by linking gene expression to the severity of the condition. While the study showcases promising advancements in using archival samples for RNA sequencing, it also raises questions about reproducibility due to limited data sharing. Join us for a compelling discussion that underscores the potential of integrating cutting-edge imaging techniques with genetic analysis, paving the way for new biomarkers and targeted therapies in respiratory health. Don't forget to subscribe for more fresh science insights! Link to episode page with article citation: www.scibud.media/podcast/season/2025/episode/68