BME 695L Lecture 12: Assessing Drug Efficacy and Nanotoxicity at the Single Cell Level

See references below for related reading.12.1      Introduction to measures of efficacy for nanomedicine12.1.1    for evaluation purposes, does structure/size reveal function?12.1.2    nanomedical treatment at the single cell level requires evaluation at the single cell level12.1.3    the difficulty of anything but simple functional assays (e.g. phosphorylated “functional” proteins)12.1.4    the need for assays which at least show correlation to functional activity12.2      Quantitative single cell measurements of one or more proteins per cell by flow and image/confocal cytometry12.2.1    cell surface measures of protein expression on live, single cells12.2.2    high-throughput flow cytometric screening of bioactive compounds12.2.3    challenges of measuring protein expression inside fixed, single cells12.2.4    when location is important 2D or 3D imaging is required to get spatial location of proteins inside cells (“locational proteomics” at the single-cell level)12.3      Quantitative multiparameter phospho-specific flow/image cytometry as a single-cell,structural-functional measurement12.3.1    attempts to measure "functional proteins" by detecting phosphorylation12.3.2    example of phospho-specific, multiparameter flow cytometry12.3.3    example of measuring single cell gene silencing by phospho-specific flow cytometry12.4      Quantitative measures of gene expression – the promises and the realities12.4.1    is gene expression at the single cell level really possible?12.4.2    is it even useful to measure a single gene's changes?12.4.3    gene arrays of purified cell subpopulations12.4.4    RNA amplification techniques to attempt to perform single cell gene arrays