BME 695N Lecture 18: Designing nanodelivery systems for in-vivo use

Outline:Overview – the in-vitro to ex-vivo to in-vivo paradigm In-vitro - importance of choosing suitable cell lines Ex-vivo – adding the complexity of in-vivo background while keeping the simplicity of in-vitro In-vivo - all the complexity of ex-vivo plus the “active” components of a real animalIn-vivo systems are open, “active” systems with multiple layers of complexity In-vitro and ex-vivo are mostly “closed” systems, but not absolutely What is an “open” system? Attempts to isolate open systemsLayers of complexity of in-vivo systems Human cells in nude mice – a mixture of in-vitro and in-vivo “Model” small animal systems Bbetter model larger animal systemsExamples of the in-vitro to in-vivo experimental pathway Kopelman group – multifunctional NPs for MRI and photodynamic therapy Langer group – aptamer-targeted NPs for cancer therapy in-vivo Leary group – peptide-guided NPs to human tumors in nude mice magnetic nanoparticles as MRI contrast agents in tissue phantoms