CRISPR–Cas9 Variants for High-Fidelity Genome Editing

Explore the shift from standard Cas9 nuclease—which makes double-strand breaks—to precision tools like Cas9 nickases (single-strand “nicks”) and dead Cas9 (binding without cutting). We break down how these variants reduce off-target indels and enable safer editing and gene regulation (CRISPRi/a, base/prime editor scaffolds) for high-fidelity genome engineering.