SKINPHAGE
from gazeta Valsassina Hosted by Colégio Valsassina
SKINPHAGE: Solution with bacteriophages to combat bacterial infections To combat antibiotic-resistant bacteria, this project aims to develop a phage solution for use in localized bacterial infections (e.g., skin wounds). Wastewater samples were collected, and the bacteriophages isolated and selected, namely the “Escherichia phage vB_EcoM-fFi-Eco06” bacteriophage. Its propagation and purification followed, as well as the extraction of its DNA, and the sequencing of its genome. The sequenced bacteriophage was propagated and purified, and a phage solution was prepared in which gauzes were soaked. These were tested against E. coli bacterial cultures to evaluate the POC: elimination of E. coli bacteria when in contact with the phage solution. The results validated the POC and the final product is a modular solution applicable to other types of bacteria. Ana Francisca Martins, Beatriz Garcia e Inês Braz Colégio Valsassina School, Lisbon, Portugal
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