BME 695L Lecture 4: Cell Targeting and its Evaluation

What this episode covers

See references below for related reading.4.1      Overview: targeting nanosystems to cells4.1.1    antibody targeting4.1.2    peptide targeting4.1.3    aptamer targeting4.1.4    ligand-receptor targeting4.2      Antibodies – polyclonal and monoclonal4.2.1    Where do antibodies come from – in nature?4.2.2    How do we make them in the laboratory?4.2.3    Monoclonal antibodies – some details you need to know!4.2.4    Labeling strategies4.2.5    Therapy problems with mouse monoclonal antibodies4.2.6    “Humanizing” monoclonal antibodies to reduce adverse host immune reactions4.2.7    Why antibodies may not be a good overall choices for targeting nanosystems to cells 4.3      Peptide targeting4.3.1    How does a peptide target?4.3.2    Examples of peptide targeting4.3.3    Creating new peptides by random peptide phage display libraries4.3.4    High-throughput screening of those peptide libraries4.3.5    Advantages and disadvantages of peptide targeting4.4      Aptamer targeting4.4.1    What are aptamers and how do they target?4.4.2    Some different types of aptamers4.4.3    How do you make aptamers?4.4.4    How do you screen for useful aptamers?4.5      Ligand-receptor targeting4.5.1    What are ligands?4.5.2    What are their advantages/disadvantages?4.5.3    Example – folate receptors4.6      How do we quantitatively evaluate targeting?4.6.1    Technologies for evaluating targeting         4.6.1.1 Flow cytometry         4.6.1.2 Scanning image cytometry4.6.2    Evaluating targeting specificity4.6.3    Evaluating targeting sensitivity

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