BME 695N Lecture 5: Cell Targeting

Outline:Overview: targeting nanosystems to cellsAntibody targetingPeptide targetingAptamer targetingAntibodies – polyclonal and monoclonalWhere do antibodies come from – in nature?How do we make them in the laboratory?Monoclonal antibodiesTherapy problems with mouse monoclonal antibodies“Humanizing” monoclonal antibodies to reduce adverse host immune reactionsWhy antibodies may not be a good overall choices for targeting nanosystems to cellsPeptide targetingHow does a peptide target?Examples of peptide targetingCreating new peptides by random peptide phage display librariesHigh-throughput screening of those peptide librariesAdvantages and disadvantages of peptide targetingAptamer targetingWhat are aptamers and how do they target?Some different types of aptamersHow do you make aptamers?How do you screen for useful aptamers?